Figure 3
Respiratory growth, oxygen consumption rates and ATP synthase activities in Δmco10, Δatp19 and Δatp19Δmco10 mutants. (a) Respiratory growth phenotypes. Cells from the indicated strains grown in glucose pre-cultures were serially diluted and spotted on rich glucose or glycerol plates with or without oligomycin (1 μg/mL) and incubated at 28 or 36 °C. The glycerol plates were photographed after three days while the glycerol + oligomycin plates after four days of incubation. (b, c, d) Oxygen consumption rates, ATP synthesis and hydrolysis activities. Measurements were performed on freshly isolated mitochondria. The state 4, state 3 and maximal oxygen consumption were measured when NADH, ADP and CCCP were added. The respiration rate is expressed in percentage of the uncoupled respiration of wild type mitochondria. ATP synthesis and hydrolysis activities on panels (c) and (d) (darker rectangles) are expressed in percentage with respect to wild type mitochondria, whereas the activities in the presence of oligomycin (at 2.5 μg/mL, lighter rectangles) are expressed as the percentage of corresponding activities without oligomycin. (e) The oxygen consumption rate of isolated mitochondria measured in the presence of 0.5, 1 or 2.5 μg/mL of oligomycin before addition of ADP. (f) ATP synthesis measured in the conditions of experiment shown in panel (e), represented as the percentage of the ATP production in control mitochondria. Data are presented from three repetitions, statistical significance is indicated by *p ≤ 0.05; **p ≤ 0.005; ***p ≤ 0.001.
