Figure 8 | Scientific Reports

Figure 8

From: Isolation, characterization, proteome, miRNAome, and the embryotrophic effects of chicken egg yolk nanovesicles (vitellovesicles)

Figure 8

Relative quantitative analysis (RT-qPCR) of mRNA transcripts expressed in the embryos treated with VVs. Five blastocysts from each group of four replicates were used for qPCR analysis. The means were normalized to the control group and expressed as arbitrary units (fold change). Data are expressed as mean ± SD and the difference between the two groups was compared using Student’s t-test. Values denoted by an asterisk (*) are considered statistically significant (p < 0.05). House-keeping genes ACTB and U6 were used for quantification of the target transcripts through the ΔΔCt method.

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