Figure 2

Principal component analysis (PCA) and correlation analysis of selected microRNAs (miR-148b-5p, miR-203, miR-342-3p, miR376a, miR-429, miR-494, miR-598, miR-697, miR-712, miR-763, miR-1903) in the electrical post-SE model. Illustration of the PCA of behavioral and biochemical parameters (a) and of expression data from selected microRNAs (b). Correlation matrix of the selected microRNAs and behavioral and biochemical parameters (c): the heat map illustrates the correlation (Spearman correlation coefficient) between the different parameters. The analysis identified a correlation between six microRNAs and weight gain in the early phase of epileptogenesis (positive correlation, blue) and with social interaction, saccharin preference and BDNF in the phase of epilepsy manifestation (negative correlation, red). All abbreviations are described in the Supplementary file. naïve naïve controls (n = 5), sham sham controls (n = 5), SE experimental animals with seizure history (n = 6), NB nestbuilding activity, BUR burrowing behavior, SI time animals spent in active social interaction, SP_percentage saccharin preference, OF open field, OF_distance open field distance moved in total, OF_rearing number of rearing postures in the open field, OF_immobile time the animal was immobile in the open field, OF_center time the animals spent in the center region of the open field, BWB black and white box, BWB_WB time the animals spent in the white box, BWB_entries number of transitions from black to white compartment, BWB_stretching number of stretching postures of the animal, BWB_LT latency to the first entry of the black box from the white box, EPM elevated plus maze, EPM_stretching number of stretching postures, EPM_head_dip number of the times the animal looked down from one of the open arms, EPM_closedarms time the animal spent in closed arms, EPM_openarms time the animal spent in open arms, EPM_open1_3 time the animal spent in the outer 1/3 of the open arms, Adrenal_glands weight of adrenal glands (g), BDNF brain-derived neurotrophic factor in pg/ml, Seizures_n number of seizures, Seizures_duration duration of seizures.