Figure 7

Role of PAK-dependent sensitivity parameter \(L_K\) in defining mutant dynamics in CPM cells. (a) Time series simulations of active Rac in the absence of diffusion at default value of \(L_K=5.77\) (top) showing mixed-mode oscillations (MMOs), at \(L_K=7\) (middle) showing non-oscillatory behaviour, and at \(L_K= 4.5\) (bottom) showing relaxation oscillations (ROs). (b) Migration tracks of CPM cells generated by altering only \(L_K\) while keeping other parameters fixed; this produced inactive (i.e., random) cells when \(L_K> 6\) (left), or actively migrating cells when \(L_K\lesssim 6\) (right). The lower the value of \(L_K\), the further the CPM cell typically migrated. (c) Mean instantaneous speed, (d) mean \(\alpha\)-values, and (e) normalized number of events evaluated over the entire simulated track from 20 cells from each of WT-like (\(L_K=5.77\)), S273A-like (\(L_K=7\)) and S273D-like (\(L_K= 4.5\)) CPM cells. Normalization of the number of events for each condition was done as in Fig. 6. The number of events in the S273A-like CPM cells did not vary because they all exhibited random inactive migration pattern; they were all zero and became negative when normalized. Error bars indicate SEM.