Table 7 Results for the evaluation of the developed methods by the three green chemistry tools (Method I &II).

From: Investigation of eco-friendly fluorescence quenching probes for assessment of acemetacin using silver nanoparticles and acriflavine reagent

1-Green analytical procedure index (GAPI)

Method I

Method II

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2-Analytical eco scale score

Method I

Reagents/instruments

Reagent, volume (mL)

No of Pictograms

Word sign

Penalty points

BR buffer, 1 mL

1

Danger

2

Acriflavine, 1.5 mL

2

Warning

2

Water

 

0

 

Item

Penalty points

Spectrofluorometer

 < 0.1 k w h per sample

0

Waste

No treatment

3

Occupational hazards

Analytical process hermitization

0

Total penalty points

  

\(\sum 7\)

Analytical eco scale score

  

100 − 7 = 93

Method II

Reagents/instruments

Reagent, volume (mL)

No of Pictograms

Word sign

Penalty points

AgNPs,1 mL

1

warning

1

Methanol, 9 mL

3

Danger

6

Item

Penalty points

 Spectrofluorometer

 < 0.1 k w h per sample

0

 Waste

No treatment

3

 Occupational hazards

Analytical process hermitization

0

 Total penalty points

  

\(\sum {10}\)

 Analytical eco scale score

  

100 − 10 = 90

Green certificate

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