Table 7 Results for the evaluation of the developed methods by the three green chemistry tools (Method I &II).
1-Green analytical procedure index (GAPI) | |||
Method I | Method II | ||
2-Analytical eco scale score | |||
Method I | |||
Reagents/instruments | |||
Reagent, volume (mL) | No of Pictograms | Word sign | Penalty points |
BR buffer, 1 mL | 1 | Danger | 2 |
Acriflavine, 1.5 mL | 2 | Warning | 2 |
Water | 0 | ||
Item | Penalty points | ||
Spectrofluorometer | < 0.1 k w h per sample | 0 | |
Waste | No treatment | 3 | |
Occupational hazards | Analytical process hermitization | 0 | |
Total penalty points | \(\sum 7\) | ||
Analytical eco scale score | 100 − 7 = 93 | ||
Method II | |||
Reagents/instruments | |||
Reagent, volume (mL) | No of Pictograms | Word sign | Penalty points |
AgNPs,1 mL | 1 | warning | 1 |
Methanol, 9 mL | 3 | Danger | 6 |
Item | Penalty points | ||
Spectrofluorometer | < 0.1 k w h per sample | 0 | |
Waste | No treatment | 3 | |
Occupational hazards | Analytical process hermitization | 0 | |
Total penalty points | \(\sum {10}\) | ||
Analytical eco scale score | 100 − 10 = 90 | ||
Green certificate | |||


