Figure 3
(a) Gene expression of secreted and processed KL in bone tissue 2 months after the treatment. Stripped bar represents gene expression in males and solid bar in females. Statistical significance presented as (*) for representing differences between treatment in male groups, and (#) for comparing treatments in females. (b) Effect of treatment on Fgf23 gene expression. (c) Expression analysis of Klotho down-stream gene c-Fos. (d) Effect of treatment on cell type specific gene expression. (e) Effect of treatment on bone protein matrix gene expression. Data are presented as fold change expression compared to Null treated animals. Relative quantity (RQ) ± standard error of RQ; n = 4–6; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.001 for differences between the treatment groups.
