Figure 1
Dependence of mitochondrial network on the cytoskeleton integrity. (a) Representative Airyscan-SR image of X. laevis melanocyte expressing EGFP-XTP (green: microtubules) and incubated with MitoTracker Deep Red FM (red: mitochondria). (b) Binary image of the mitochondria network obtained for the cell shown in (a). The yellow line indicates the cell contour estimated as described in Supplementary Section 4. (c) Quantification of the mitochondrial cell coverage. Measurements were performed in control cells (CTRL), cells incubated with nocodazole (NOC), latrunculin-B (LAT) or vinblastine (VINB) and cells expressing the dominant negative vimentin mutant mCherry-(vim(1-138)) (VIM\(^-\)). Each circle represents a single cell. Asterisks denote significant differences (p-value < 0.05) with respect to the control condition. The values obtained are specified in Supplementary Table S2. (d) Mitochondria and microtubule orientation analysis. Zoom-in images of the regions included in the squares (box 1–2) delimited in the cell shown in (a). Mitochondria (mito) and microtubules (MT) images were analyzed independently to compute the local orientation angle (arrows) within the same subcellular region. Both magnitudes were plotted and compared through a linear regression routine as described in “Methods”.
