Figure 2 | Scientific Reports

Figure 2

From: CRISPR-Cas9 genetic screen leads to the discovery of L-Moses, a KAT2B inhibitor that attenuates Tunicamycin-mediated neuronal cell death

Figure 2

Arrayed screen identifies 13 significant hits. (A) Diagram showing the strategy for the arrayed screen. In this simplified example, transduction efficiency is 50%. In the actual experiment, transduction efficiency varied between the different KOs. (B) Graph showing the Tun/Unt ratio of BFP positive cells. D14 neurons were treated with 100 nM Tun for 7 days. Data are shown as ± SEM, n = 6 (3 replicas from 2 independent experiments). Dotted line shows Tun/Unt = 1. One-way ANOVA with Dunnett’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 compared to the neutral control (BFP/GFP; blue bar).

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