Figure 1
Cellular inhibition assay. (a) Screening inhibition assay with 28 scFv-hFc antibodies against eqIL-5 on receptor positive cells using 1000 nM antibody and 5 nM interleukin (related to dimer) (molar ratio 200:1). The cut-off for the selection of inhibiting antibodies is marked with a red line and antibodies for further analysis are marked in colors. (b,c) Titration inhibition assay with antibodies in (b) scFv-hFc format and (c) eqIgG6 format using 1000 nM–0.32 nM antibody and 5 nM antigen (related to dimer) (molar ratio 200:1–0.064:1). IC50 values were determined with OriginPro using the Logistic5 Fit. For the isotype control a linear fit was applied. Screening and titration assays were performed in single measurements (n = 1). n.p. not producible, n.d. not determined.
