Figure 4
From: Development of novel broad-spectrum antimicrobial lipopeptides derived from plantaricin NC8 β
Secondary structure characterization and critical aggregation concentration (CAC) determination. (A) CD spectra of 30 µM L-6-C5 and L-6-C5-Leu peptides without (dotted line) and with (full line) 1.2 mM POPC:POPG (75:25) liposome models in PBS buffer (10 mM, pH 7.4). Inset: analysis of amino acid propensity of (i) L-6-C5 and (ii) L-6-C5-Leu peptide sequences (with exclusion of the fatty acid tail). (B-E) DLS analysis: scattering intensity of lipopeptides (B) L-6, (C) L-6-C2, (D) L-6-C5 and (E) L-6-C7 in 10 mM PBS (pH 7.4). (F) Relationship between critical aggregation concentration (CAC) and fatty acid chain length of the lipopeptides L-6, L-6-C2, L-6-C5 and L-6-C7.
