Table 2 Number of seeder pigs presenting ASFV viremia detectable by qPCR (Ct < 35), and number of dead pigs, over the 35 days following oronasal/intramuscular inoculation with the ASFV (challenge).
Seeder pigs | Parameters | Days post challenge | |||||||
|---|---|---|---|---|---|---|---|---|---|
0 | 5 | 7 | 10 | 14 | 21 | 28 | 35 | ||
ASF-H | No. of pigs with Ct < 35.00 | 0/3 | 2/3 | 3/3 | 0/2 | 2/2 | 1/1 | –* | – |
Average Ct value | ≥ 35.00 | 25.50 | 20.55 | 17.90 | 16.90 | 28.13 | – | – | |
SD | 0.00 | 7.20 | 1.65 | 1.00 | 0.00 | 6.22 | – | – | |
No. of dead pigs/total no | 0/3 | 0/3 | 0/3 | 1/3 | 1/3 | 2/3 | 3/3 | 3/3 | |
ASF-M | No. of pigs with Ct < 35.00 | 0/3 | 2/3 | 3/3 | 2/3 | 1/2 | 1/1 | – | – |
Average Ct value | ≥ 35.00 | 32.90 | 31.87 | 30.50 | 20.55 | 17.00 | – | – | |
SD | 0.00 | 3.33 | 0.29 | 1.93 | 1.65 | 0.00 | – | – | |
No. of dead pigs/total no | 0/3 | 0/3 | 0/3 | 0/3 | 1/3 | 2/3 | 3/3 | 3/3 | |
ASF-L | No. of pigs with Ct < 35.00 | 0/3 | 1/3 | 1/3 | 2/3 | 1/3 | 2/3 | 1/3 | 2/2 |
Average Ct value | ≥ 35.00 | > 35.00 | > 35.00 | > 35.00 | > 35.00 | > 35.00 | > 35.00 | 22.67 | |
SD | 0.00 | 1.42 | 1.09 | 2.73 | 0.64 | 0.49 | 1.00 | 1.82 | |
No. of dead pigs/total no | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 1/3 | |
Negative control | No. of pigs with Ct < 35.00 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
Average Ct value | ≥ 35.00 | ≥ 35.00 | ≥ 35.00 | ≥ 35.00 | ≥ 35.00 | ≥ 35.00 | ≥ 35.00 | ≥ 35.00 | |
SD | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |
No. of dead pigs/total no | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | |
