Figure 2 | Scientific Reports

Figure 2

From: Extracellular vesicles released from ganglioside GD2-expressing melanoma cells enhance the malignant properties of GD2-negative melanomas

Figure 2

Immunoblotting of EV markers and a lipid raft marker. Immunoblotting of cell lysates and EVs from GD2+ S1 cells and GD2- V4 cells was performed using specific mAbs for the individual molecules. Anti-GD2 and anti-β-actin antibodies were also used (A). The results of immunoblotting with anti-CD63 are shown separately in ( B). Lysates from GD2+ S1 and GD2- V4 cells and lysates from S1- and V4-derived exosomes were subjected to SDS‒PAGE. Subsequently, immunoblotting was performed using antibodies reactive with EV markers, e.g., CD9, CD81, Tsg101, CD63, a lipid raft marker Flotillin-1 (all used at 1:1000 dilution), GD2 (1:100 dilution of ascites), and β-actin (1:1000 dilution) and GAPDH (1:1000 dilution). An ImmunoStar LD detection kit was used for the detection of bands using Amersham Imager (Model: 680 software version 2.0, GE Healthcare, Uppsala, Sweden). These results are the representative data of repeated experiments with similar patterns.

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