Figure 4 | Scientific Reports

Figure 4

From: Extracellular vesicles released from ganglioside GD2-expressing melanoma cells enhance the malignant properties of GD2-negative melanomas

Figure 4

Effects of EVs on the adhesion of GD2- and GD2 + cells. (A, B) Cell adhesion was investigated using GD2- V4 cells and GD2+ S1 cells in collagen I-precoated (5 μg/mL in PBS, 100 μL/well) 16-well microplates by an RT-CES system. Cells (1 × 104) were seeded in each well of the plates containing 100 μl of culture medium, EVs (0.5, 2.0 and 4.0 μg) derived from S1 cells were added to V4 cells at 0 h (A), and EVs (0.5, 2.0 and 4.0 μg) derived from V4 cells were added to S1 cells at 0 h (B). PBS was added to control cells. The data at 3 h and 24 h incubation (A) or at 7.5 h and 24 h (B) were analyzed with an unpaired Student’s two-tailed t test. **P < 0.01 and ***P < 0.001.

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