Figure 1

Experimental set-up for comparison of tear fluid pre-processing methods, and protein distribution on a Schirmer strip using PEA technology by Olink Proteomics. Tear fluid from 5 healthy volunteers was collected with Schirmer strips. Strips were cut vertically into two equal portions. Out of one part of the strip, four 1.2 mm punches were taken from various locations of the strip (A: head; B: 0–10 mm; C: 10–20 mm; D: 20–30 mm). The other part of the strip was cut into horizontal locations (A: head; B: 0–10 mm; C: 10–20 mm; D: 20–30 mm) and subsequently the locations were eluted in 60 µL buffer. The punches as well as 1 out of 40 µl of the eluates were supplied to Olink Proteomics in a 96 wells plate.