Figure 2 | Scientific Reports

Figure 2

From: Embryo morphokinetics derived from fresh and vitrified bovine oocytes predict blastocyst development and nuclear abnormalities

Figure 2

Cleavage and day 8 blastocyst rate over zygotes starting in vitro culture after 21 h of in vitro fertilization. Different superscripts (*, **; a, b;+, ++; d, e) per group of bars represent statistical differences (p < 0.05). Results are stated as least square means ± standard errors. Control: fresh cumulus enclosed oocytes. Corona radiata (CR): fresh partially denuded oocytes enclosed by the CR only. VCR-H: CR oocytes vitrified with a protocol using high concentrations of cryoprotectants (15%) in equilibration solution. VCR-L: CR oocytes vitrified with a protocol using a low cryoprotectant (3%) concentration in equilibration solution. Numbers of zygotes in group culture (Control: n = 582, CR = 369, VCR-L = 183, VCR-H = 239) and in Well-of the-well (WOW) time-lapse (Control = 127, CR = 94, VCR-L = 91, VCR-H = 91).

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