Table 5 Percentage of vitrified and fresh zygotes that reach each division.

From: Embryo morphokinetics derived from fresh and vitrified bovine oocytes predict blastocyst development and nuclear abnormalities

Treatment

n

First division

Second division

Third division

Fourth division

Control

313

78.0 ± 2.5

88.6 ± 2.5a

76.6 ± 3.4a

51.7 ± 4.0a

CR

82

90.3 ± 3.3

87.0 ± 4.5ab

61.7 ± 3.4ab

32.9 ± 6.3b

VCR-L

56

78.6 ± 5.5

79.4 ± 6.3ab

50.7 ± 2.0b

15.6 ± 5.4bc

VCR-H

63

81.0 ± 4.9

72.6 ± 7.0b

43.6 ± 8.1b

7.4 ± 3.7c

  1. n = number of zygotes/embryos analyzed. Percentage of zygotes reaching different divisions. Different superscripts (a, b, and c) per column represent statistical differences (p < 0.05). Results are stated as least square means ± standard errors. All oocytes were cultured in the Well-of the-well (WOW) system. Control: fresh cumulus enclosed oocytes. Corona Radiata (CR): fresh oocytes partially denuded to leave only the CR. VCR-H: CR oocytes vitrified with a protocol using high concentrations of cryoprotectants (15%) in equilibration solution; VCR-L: CR oocytes vitrified with a protocol using a low cryoprotectant concentration (3%) in equilibration solution.
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