Table 6 Proportion of morphokinetic events around cleavage or first division.

From: Embryo morphokinetics derived from fresh and vitrified bovine oocytes predict blastocyst development and nuclear abnormalities

Treatment

n

Slow cleavers

Lag-phase

Multipolar division

Ruffling membrane

Uneven blastomere area

Control

313

54.3 ± 2.6a

43.5 ± 2.8a

21.9 ± 2.5

15.7 ± 2.0

38.9 ± 3.4

CR

82

72.0 ± 5.2b

26.8 ± 4.8b

9.7 ± 3.3

17.1 ± 4.1

28.3 ± 5.4

VCR-L

56

75.0 ± 6.3b

10.7 ± 4.1bc

21.4 ± 5.5

10.7 ± 4.1

46.6 ± 7.2

VCR-H

63

82.5 ± 5.9b

4.7 ± 2.7c

21.4 ± 5.5

19.0 ± 4.9

49.3 ± 6.9

  1. Percentage of zygotes that display abnormal events around the first division. Slow cleavers are considered zygotes that cleave after 32 h and 22 min. Uneven blastomere are indicates a difference between blastomere areas of more than 23.4%. Different superscripts per column (a, b, and c) represent statistical differences (p < 0.05). Results are expressed as least square means ± standard errors. Control: fresh cumulus enclosed oocytes. Corona radiata (CR): fresh oocytes partially denuded to leave only the corona radiata. VCR-H: CR oocytes vitrified with a protocol using high concentrations of cryoprotectants (15%) in equilibration solution; VCR-L: CR oocytes vitrified with a protocol using a low cryoprotectant concentration (3%) in equilibration solution.
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