Table 3 Primers used for PCR analysis.
From: The first detection of two Aeromonas strains in mice of the genus Apodemus
Primer | Target gene | Nucleotide sequence (5′ → 3′) | Amplicon size (bp) | Annealing T, °C |
|---|---|---|---|---|
CphA-F | cphA | TGGTGCTGRTGGCGAGTT | 346 | 56 |
CphA-R | GCCCARTCGCTCTTCATCA | |||
IntI1-F | intI1 | GTTCGGTCAAGGTTCTG | 920 | 50 |
IntI1-R | GCCAACTTTCAGCACATG | |||
InF | Variable region | GGCATACAAGCAGCAAGC | Variable | 50 |
InB | AAGCAGACTTGACCTGAT | |||
hlyA-F | hlyA | CCACGCAAATTCATCACG | 1 079 | 55 |
hlyA-R | ATCCTTGTTCACCTCGAC | |||
alt-F | alt | TGACCCAGTCCTGGCACGGC | 442 | 55 |
alt-R | GGTGATCGATCACCACCAGC | |||
ast-F | ast | TCTCCATGCTTCCCTTCCACT | 331 | 55 |
ast-R | GTGTAGGGATTGAAGAAGCCG |
