Figure 6
From: The impact of pericytes on the stability of microvascular networks in response to nanoparticles
The impact of cationic nanoparticles on vascular network integrity. (A) Following 4-day treatment with cationic silica nanoparticles, a significant reduction in vessel diameter is observed in HUVEC monocultures. This is not observed when HUVECs are co-cultured with pericytes. (B) Representative images of microvascular networks following treatment with cationic silica nanoparticles, z-projection images were generated using confocal images. Red, F-actin. Green, CD31. Scale bar: 100 μm. (C–E) Quantification of relative mean intensity for cleaved caspase 3 (ratio treated/Ctrl) and cytotoxicity via ethidium homodimer-1 (ratio treated/Ctrl) and mean intensity of perivascular fibronectin (FN) deposition. N = 3; n.s., non significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.
