Table 1 Spatial and temporal replicates included in the eDNA metabarcoding study of Rana temporaria in the Province of Trento, Italy.

From: A validated protocol for eDNA-based monitoring of within-species genetic diversity in a pond-breeding amphibian

Site

T1

T2

T3

S1

S2

S3

Repl. T1

S1

S2

s3

Repl. T2

S1

S2

S3

Repl. T3

Tot Repl

Amp

x

nd

x

2/3

nd

x

x

2/3

nd

nd

nd

0/3

4/9

Bed

x

x

/

2/2

x

x

/

2/2

nd

nd

/

0/2

4/6

Fia

x

x

x

3/3

x

x

nd

2/3

nd

nd

nd

0/3

5/9

Ing

x

x

nd

2/3

x

nd

nd

1/3

nd

nd

nd

0/3

3/9

Lel

x

nd

/

1/2

nd

/

/

0/1

/

/

/

0/0

1/3

Pla

x

x

x

3/3

nd

nd

nd

0/3

nd

nd

nd

0/3

3/9

PMa

nd

x

x

2/3

nd

nd

nd

0/3

nd

nd

nd

0/3

2/9

PS1

x

x

/

2/2

x

nd

/

1/2

nd

nd

/

0/2

3/6

PS2

x

x

/

2/2

nd

nd

/

0/2

nd

nd

/

0/2

2/6

Va1

x

x

/

2/2

x

x

/

2/2

x

nd

/

1/2

5/6

            

TOT

32/72

  1. T1, T2, T3 = temporal replicates 1, 2, and 3; Repl. T1, Repl. T2, Repl. T3: for every temporal point, the sum of spatial replicates per site is reported (positive/total); S1, S2, S3 = spatial replicates 1, 2, and 3; x = sampling was performed and positive results were obtained after sequencing; nd = sampling was performed but amplification was not successful (no DNA bands visible on the Qiaxcel Advanced System (Qiagen) and/or several samples were sequenced for confirmation and no R. temporaria DNA was detected); / = sampling was not performed because the structure of the wetland site was not compatible with three spatial replicates (see text for details) or, (for T3) one of the previously selected water microhabitats was no longer physically accessible or non-existent (due to seasonal changes in wetland extent and hydrology).
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