Figure 3
Cell spreading ability in freshly harvested and freeze-thawed human clonal BMSCs. (a) Phase contrast images of freshly harvested and freeze-thawed clonal BMSCs at 15-, 30-, and 60-min incubation after seeding in culture. Scale bars represent 200 μm. (b) Adherent cell numbers of freshly harvested (orange) and freeze-thawed (blue) clonal BMSCs are shown. Data shown are mean ± SD (n = 4). Statistical significance: Student’s t-test, not significant (N.S.), **P < 0.01. (c) Gene expression of ITGB1 in freshly harvested (orange) and freeze-thawed (blue) clonal BMSCs were shown. Data shown are mean ± SD (n = 4). Statistical significance: Student’s t-test, not significant (N.S.).
