Fig.1 | Scientific Reports

Fig.1

From: Anti-proliferative effect of melatonin in human hepatoma HepG2 cells occurs mainly through cell cycle arrest and inflammation inhibition

Fig.1

The anti-proliferative activity of melatonin toward HCC cells. (A) Dose–response curve shows the cytotoxic effect of melatonin on wild HepG2 cells viability using MTT assay. Wild HCC cells (HepG2) were treated with various concentrations of melatonin (0–10 mM) for 24 h, 48 h, and 72 h. Melatonin was found to reduce the % viability of HepG2 cells in a time and concentration-dependent manner. GraphPad Prism version 8.0 (non-linear regression model) was used to calculate IC50 values of melatonin for each time interval. The experiment was performed in octuplets. (B) A column chart shows the calculated IC50 doses of melatonin for each incubation time (24 h, 48 h, and 72 h). Data are presented as mean ± SEM. “a” represents p < 0.01, and “b” represents p < 0.001, as compared to control.

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