Figure 4

The inhibitory effect of 1 mM melatonin on the inflammation. a. Human inflammation antibody array membranes detect signal spots of 40 targets/membrane. (A) The array membrane of vehicle control (DMSO treated) HepG2 cells, (B) The array membrane of 1 mM melatonin-treated HepG2 cells for 24 h. After development of the membranes, the imaging of the spots signals' was detected by Chemiluminescence using CCD camera of chemiluminescence imager (UVP, UK), and the raw densitometry data was measured with the Image Studio Lite software, (https://www.licor.com/), b. The anti-inflammatory effect of 1 mM melatonin. The graph shows the effect of 1 mM melatonin for 24 h on the mean expression levels of normalized inflammatory mediators in HepG2 cells, in a comparison to the control untreated cells. After subtracting the background signals, each spot was normalized to the positive control before comparing analyte-by-analyte to determine relative differences in inflammatory factors expression for each sample. All cytokines were expressed in non-significant varied lower levels as compared to control untreated HepG2 cells. The data were calculated as a mean of duplicate ± SEM, and c. the calculated fold changes (of 40 inflammatory factors expressed in 1 mM melatonin-treated HCC-HepG2 cells after incubation time of 24 h) to control. The chart displays the decreasing of the expression levels of all detected inflammatory mediators with the lowest expression levels were calculated for interleukin (IL)-7, and IL-13.