Figure 2
From: Hiat1 as a new transporter involved in ammonia regulation
Analysis of Carcinus maenas Hiat1 mRNA abundance by quantitative PCR. (A) Organ and tissue mRNA abundance profile of Carcinus maenas Hiat1 (black bars); (B) Short-term (24 h) acclimation of green crabs to high environmental ammonia of 1 mmol L−1 (HEA). Grey bars referred to on the left y-axis in panel (B) represent mRNA abundance of Carcinus maenas Hiat1 in posterior gill #7 at 0, 6, and 24 h of HEA acclimation. Line graph referred to on the right y-axis in panel (B) represents fold change in hemolymph ammonia levels after 0, 2, 6, and 24 h of HEA acclimation. Carcinus maenas Hiat1 has been normalized to either Ribosomal gene 3 (Rbs-3, panel (A)) or elongation factor 1α (Ef1α, panel (B)). Upper case letters denote significant differences in mRNA abundance, lower case italic letters denote significant changes in hemolymph ammonia (ANOVA with Tukey’s post-hoc analysis, P > 0.05, Ntissue = 5; NHemoAmm = 6; NRNA,0 h = 6, N RNA,6 h = 7, N RNA,24 h = 8). Values are presented as means ± SE. Cer.gang., cerebral ganglion; Hypoderm., hypodermis; Ant. gland, antennal gland; Ant. gill, anterior respiratory gill #5; Post. gill, posterior osmoregulatory active gill #7.
