Figure 4

SIET measurements of net fluxes in Xenopus laevis oocytes expressing Carcinus maenas Hiat1. (A) NH₄⁺ uptake (positive values, bath to oocyte) and (B) H⁺ release (negative values, oocyte to bath) kinetics of sham-injected (dotted line, empty circles) and Carcinus maenas Hiat1-expressing (solid line, black squares) oocytes in baths with increasing NH₄Cl concentration. Asterisks in (A, B) denote significant differences between sham-injected and Carcinus maenas Hiat1-expressing oocytes with regards to either NH₄⁺ or H⁺ flux at the given bath [NH₄Cl] (two-way ANOVA with Bonferroni multiple comparisons, P < 0.05, N = 5). Values are presented as means ± SE. Experiments have been conducted on two different batch of oocytes (i.e., different female, different days).