Figure 1
Aromatase inhibitors induce mechanical allodynia, reduce the muscle strength of mice and increase the kinin B1 and B2 receptor protein expression. Time-response curves to the mechanical allodynia (A,C) and the muscle strength at 3 h (B,D) after vehicle (10 mL/kg, p.o.), anastrozole (0.2 mg/kg, p.o. A,B), or letrozole (0.5 mg/kg, p.o. C,D) administration. (B) Denotes baseline mechanical threshold or muscle strength before drug administrations. Representative imagens of kinin B1 receptor protein expression in the plantar tissue (E,F), kinin B2 receptor protein expression in sciatic nerve (G,H) and spinal cord (I,J) at 3 h after vehicle (10 mL/kg, p.o.), anastrozole (0.2 mg/kg, p.o.) or letrozole (0.5 mg/kg, p.o.) administration. Data are expressed as the mean + SEM. *P < 0.05; **P < 0.01; ***P < 0.001 when compared to the vehicle group; two-way repeated-measures ANOVA followed by Bonferroni’s post hoc test to the behavioral tests (n = 6/group; C57BL/6 mice) or Student’s t-test to the protein expression (n = 4–7/group; C57BL/6 mice). PWT paw withdrawal threshold. The original western blot images are available in Supplementary Fig. S2.
