Figure 4
From: Development of anti-feline PD-1 antibody and its functional analysis

PD-1 and PD-L1 expression in stimulated feline lymphocytes. Peripheral blood lymphocytes were isolated from the peripheral blood and stimulated with 10 µg/mL of concanavalin A (Con A) for 48 h. After culture, the cells were collected and stained with anti-PD1 (1A1-2) mAb or anti-PD-L1 (G11-6) mAb, followed by anti-mouse IgG-alexa647 or anti-rat IgG-DyLight649 antibodies, respectively.