Figure 5
From: Development of anti-feline PD-1 antibody and its functional analysis
Augmentation of IFN-γ production in concanavalin A (Con A)-stimulated peripheral blood lymphocytes (PBLs) treatment with anti-PD-1 mAb or anti-PD-L1 mAb. PBLs were isolated from seven healthy cats and stimulated with 10 µg/mL of Con A for 48 h in the presence of mouse IgG1 isotype control or anti-fPD1 (1A1-2) mAb (A), rat IgG2a isotype control or anti-fPDL1 (G11-6) mAb (B). After 48 h, the culture supernatant was collected and the amount of feline IFN-γ in the supernatants was measured in duplicate. Different color bars indicate different individuals.
