Figure 5
Increased cholesterol biosynthesis pathway in lung cancer cells caused by the overexpression of HMGCR and ACAT1 in the tumoral lung region of patients with LUAD and qPCR analysis of relative ACAT1/RPL30, HMGCR/RPL30 and ABCA1/HPRT mRNA expression level in patients with LUAD. (a) Cholesterol biosynthesis pathway and the accumulation of cholesterol caused by an overexpression of ACAT1 in cancer cells. (b) qPCR analysis of relative HMGCR/RPL30 mRNA expression level, measured in the control region and tumoral region of patients with LUAD (CTR:n = 33; TU: n = 32). (c) qPCR analysis of HMGCR/RPL30 mRNA expression level subdivided into tumor gradings (CTR G1: n = 5; CTR G2: n = 14; CTR G3: n = 14; TU G1: n = 5; TU G2: n = 14; TU G3: n = 13). (d) qPCR analysis of relative ACAT1/RPL30 mRNA expression level in patients with LUAD: control region (CTR) and tumoral region (TU). (CTR n = 32; TU n = 30, p (CTR vs. TU) = < 0.0001). (e) qPCR analysis of ACAT1/RPL30 mRNA expression level subdivided into tumor gradings (CTR G1:n = 5; CTR G2: n = 14; CTR G3: n = 13; TU G1: n = 4; TU G2: n = 14; TU G3: n = 12, p (CTR G2 vs. TU G2) = 0.0125). (f) qPCR analysis of relative ACAT1/RPL30 mRNA expression level measured in the control region correlated with relative ACAT1/RPL30 mRNA expression level measured in tumoral region of patients with LUAD (n = 30, p = 0.1440, R2 = 0.0386). (g) qPCR analysis of relative ACAT1/RPL30 mRNA expression level measured in the control region (CTR) correlated with total cholesterol values of LUAD patients (n = 25, p = 0.240, R2 = 0.2026). (h) qPCR analysis of relative ACAT1/RPL30 mRNA expression level measured in the tumoral region (TU) correlated with total cholesterol values of LUAD patients (n = 24, p = 0.6297, R2 = 0.01075). (i) qPCR analysis of relative ABCA1/HPRT mRNA expression level, measured in the control region and tumoral region of patients with LUAD (CTR:n = 33; TU: n = 32). (j) qPCR analysis of ABCA1/HPRT mRNA expression level subdivided into tumor gradings (CTR G1:n = 5; CTR G2: n = 14; CTR G3: n = 13; TU G1: n = 3; TU G2: n = 14; TU G3: n = 12, p (CTR G3 vs. TU G3) = 0.0085). Correlations are shown by using simple linear regression. Paired t-test was performed for Figure b,d and i; Kruskal Wallis test was performed for Figure e and j. Data are shown as mean values ± s.e.m.; *P < 0.05; **P < 0.01***P < 0.001 ****P < 0.0001.
