Figure 6

Using CAHS D and engineered CAHS D variants as potential stabilizers of FVIII under thermal stress. Histograms of 50% clotting time of FVIII treated with (A) lysozyme, (B) CAHS D, (C) CAHS D Linker Region, or (D) CAHS D 2X Linker prior to desiccation and thermal stress. The red “FVIII (+)” bar represents FVIII deficient human blood plasma treated with non-perturbed FVIII. All red notations above sample bars represent statistical comparison with “FVIII (+).” “X mg/mL'' notation beneath sample bars correspond to the concentration of protein mixed with FVIII before desiccation. All pink notations above sample bars represent statistical comparison with “0 mg/mL.” The green “FVIII (−)” bar represents FVIII deficient human blood plasma not treated with FVIII. “No FVIII + X mg/mL protein” represents FVIII deficient human blood plasma treated with X mg/mL protein, but no FVIII. All purple notations above sample bars represent statistical comparison with “No FVIII + X mg/mL protein.” “NA” denotes that, although the relevant experiment was conducted, no clotting was observed during the entire duration of the clotting assay. Notations above sample bars represent statistical significance determined by one-way ANOVA and Tukey’s post- hoc test. Error bars represent bi-directional standard deviation. P value > 0.05 = NS, P < 0.05 = *, P < 0.01 = **, P < 0.001 = ***.