Figure 5
From: Mechanism of ferroptosis in a rat model of premature ovarian insufficiency induced by cisplatin
Protein and RNA expression level changes of ferroptosis related molecules in ovarian tissues and GCs of each group. (A, D) Western blot bands of ferroptosis related molecules in ovarian tissue and GCs. One representative blot from three independent experiments is shown, β-actin was used as the loading control. (B) Quantitation on protein expression of ACSL4, ALOX15, SLC7A11, and GPX4 in ovarian tissues. (C) The RNA expression of ACSL4, ALOX15, SLC7A11 , and GPX4 by qRT-PCR analysis in ovarian tissues. (E) Quantitation on protein expression of ACSL4, ALOX15, SLC7A11 in GCs. (F) The RNA expression of ACSL4, ALOX15, SLC7A11 by qRT-PCR analysis in GCs. 18S was used as an internal control. Data are expressed as the means ± SEM. ns, * versus control, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001, ns indicates no statistical significance. N, # versus CDDP + VE, # P < 0.05, ## P < 0.01, ###P < 0.001 and #### P < 0.0001, N indicates no statistical significance.
