Figure 2 | Scientific Reports

Figure 2

From: Development of loop-mediated isothermal amplification (LAMP) assays using five primers reduces the false-positive rate in COVID-19 diagnosis

Figure 2

(a) Colorimetric RT-LAMP using E-ID1 primer set on clinical samples before and after optimization. The optimized colorimetric assay started to detect the positive samples within 30 min compared to 50 min before optimization. (b) Fluorometric RT-LAMP before (cycle number on the x-axis also corresponds to reaction time, in minutes, since the reaction set up here was 60 cycles of 1 min) and (c) after optimization using LavaLAMP reagents (Lucigen, Middleton, WI, USA) on different clinical samples, where the improvement in the detection time can be evident.

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