Table 1 Sequences of each RT-LAMP primer set used in this study.

From: Development of loop-mediated isothermal amplification (LAMP) assays using five primers reduces the false-positive rate in COVID-19 diagnosis

Primer set

Sequence (5′–3′)

N-ID15

F3: AGATCACATTGGCACCCG

B3: CCATTGCCAGCCATTCTAGC

FIP: TGCTCCCTTCTGCGTAGAAGCCAATGCTGCAATCGTGCTAC

BIP: GGCGGCAGTCAAGCCTCTTCCCTACTGCTGCCTGGAGTT

LF: GGCAATGTTGTTCCTTGAGGAAGTT

LB: CACGTAGTCGCAACAGTTCAA

N-ID15n1L*

F3: AGATCACATTGGCACCCG

B3: CCATTGCCAGCCATTCTAGC

FIP: TGCTCCCTTCTGCGTAGAAGCCAATGCTGCAATCGTGCTAC

BIP: GGCGGCAGTCAAGCCTCTTCCCTACTGCTGCCTGGAGTT

LF: GCAATGTTGTTCCTTGAGGAAGTT

LB: GTTCCTCATCACGTAGTCGCAACA

S-ID17

F3: TCTTTCACACGTGGTGTT

B3: GTACCAAAAATCCAGCCTC

FIP: CATGGAACCAAGTAACATTGGAAAACCTGACAAAGTTTTCAGATCC

BIP: CTCTGGGACCAATGGTACTAAGAGGACTTCTCAGTGGAAGCA

LF: GGTAAGAACAAGTCCTGAGTTGAA

LB: GTTTGATAACCCTGTCCTACCATT

S-ID24

F3: GGTGTTTATTACCCTGACAAAG

B3: GTACCAAAAATCCAGCCTC

FIP: CATGGAACCAAGTAACATTGGAAAATTTTCAGATCCTCAGTTTTACATTC

BIP: CTCTGGGACCAATGGTACTAAGAGGACTTCTCAGTGGAAGCA

LF: GAAAGGTAAGAACAAGTCCTGAGT

LB: GTTTGATAACCCTGTCCTACCATT

E-ID1*

F3: TCATTCGTTTCGGAAGAGA

B3: AGGAACTCTAGAAGAATTCAGAT

FIP: TGTAACTAGCAAGAATACCACGAAACAGGTACGTTAATAGTTAATAGCG

BIP: GCTTCGATTGTGTGCGTACTCGAGAGTAAACGTAAAAAGAAGG

LB: GCTGCAATATTGTTAACGTGAGTC

RdRp-ID37

F3: ACAAAGCCTTACATTAAGTGG

B3: CACCATCAACAAATATTTTTCTCAC

FIP: TGGGTGGTATGTCTGATCCCAATAGATTTGTTAAAATATGACTTCACGG

BIP: TGTGTTAACTGTTTGGATGACAGATTGTAAGTGGGAACACTGT

LF: ACGGTCAAAGAGTTTTAACCTCTCT

LB: GCATTCTGCATTGTGCAAACT

  1. F3, forward outer; FIP, forward inner; LF, loop forward; B3, backward outer; BIP, backward inner; LB, loop backward. All the primers were designed by using the PrimerExplorer program. *Jamwal et al.27 designed and used these primer sets.
Back to article page