Figure 3
MTT cell viability assay of ORF3a transfected cells to investigate inhibitory action on ORF3a. (A) HEK293 cells were transfected with empty vector (control) and treated with the indicated inhibitors. Compounds did not affect cell viability. (B) Quercetin, EGCG and nobiletin at concentrations of 5 µM (Que), 10–20 µM (EGCG, Que and Nob) led to increased cell viability, indicating proliferative activity. (C) Viability test of viroporin inhibitors candidates. HEK293 cells were transfected with ORF3a and treated with increasing concentrations of inhibitor. For each data set, the first bar represents the control value (= 1) (white), then relative cell viability after expression ORF3a (black), followed by normalized viability readings at increasing concentrations of inhibitor (grey). (A–C) Significance relative to ORF3a-transfected cells in absence of inhibitor was determined using one-way ANOVA with * = p < 0.05; ** = p < 0.01, no sign = not significant. Abbreviations are as follows: Rim = rimantadine, Ama = amantadine, EGCG = epigallocatechin gallate, Que = quercetin, Nob = nobiletin, Res = resveratrol, Kae = kaempferol, Cur = curcumin, Api = apigenin, Gen = genistein, Nar = naringenin, Gin = 6-gingerol.
