Figure 4
Concentration–response analysis of viroporin inhibition. (A, B) Concentration–response curves. ORF3a activity was calculated from viability data (see Methods), and normalized to viability data in absence of inhibitor. Note that in cases, where cell viability of treated ORF3a expressing HEK293 cells was increased as compared to control, relative absorbances could reach values below 0 in the IC50 graph. (A) Classical viroporin inhibitors; solid squares = rimantadine, solid circles = amantadine (B) Test compounds; solid squares = EGCG, solid circles = quercetin, up triangles = nobiletin, down triangles = resveratrol, diamonds = kaempferol, crossed squares = curcumin. (C) IC50 values of classical inhibitors and flavonoids. Abbreviations are as in Fig. 3. (D) Percentage inhibition was obtained from MTT data at the highest inhibitor concentration. All active inhibitors achieved complete inhibition (~ 100%) except amantadine (78%), curcumin (72%) and resveratrol (86%).
