Figure 5
Patch-Clamp Electrophysiology measurements from HEK293 cells expressing SP-ORF3a. (A) IV ramp form − 60 mV to 50 mV was applied to mock-transfected cells and compared to IV ramps from cell transfected with SP-ORF3a in the absence and presence of inhibitors. Currents from ORF3a-expressing cells were increased compared to mock-transfected control cells. Currents were reduced and reached control values upon treatment with classical inhibitors (rimantadine and amantadine) or phenolics. Vertical and horizontal scale bars denote 0.5 nA and 10 ms, respectively. EG = epigallocatechin gallate. (B) Current–voltage relationship of all tested inhibitors. Open circles = mock transfected HEK293 cells; squares = ORF3a expressing cells without inhibitor; crossed squares = small inhibitor concentration (0.5–1 nM), crossed square = intermediate inhibitor concentration (1–5 nM); open squares = highest inhibitor concentration (20–50 nM). (C) IC50 curves from patch-clamp inhibition data: solid squares = rimantadine, solid circles = amantadine, open squares = EGCG, open circles = quercetin, up-triangles = nobiletin, down-triangles = kaempferol, diamonds = curcumin, crossed squares = resveratrol, dotted squares = 6-gingerol. (D) Summary of IC50 values from patch-clamp electrophysiology. Abbreviations are as listed in Fig. 3.
