Figure 2 | Scientific Reports

Figure 2

From: Differentiation of patient-specific void urine-derived human induced pluripotent stem cells to fibroblasts and skeletal muscle myocytes

Figure 2

Characterization of urine cell-derived iPSC line U1. Urine cells were reprogrammed to iPSCs using the Epi5 reprogramming system. (A–G) Immunocytochemistry of episome-free U1 line confirms the expression of pluripotency markers in the newly derived iPSC line U1: (A) phase contrast image of a U1 iPSC colony grown on Geltrex in mTeSR, and corresponding co-immunolabeling for (B) DAPI, (C) OCT4, and (D) SOX2. (E) Phase contrast, and co-immunolabeling for (F) DAPI, and (G) NANOG. Bar: 100 µm. (H) G-banded analysis of U1 metaphase chromosomes reveals a normal female karyotype. (I, J, K) Teratoma formation assay of undifferentiated U1 injected into kidney capsule of immune deficient athymic nude mice. Immunohistochemistry of dissected tumors shows tri-lineage differentiation into TUBB3 positive neuroectoderm (I, brown staining), hepatocyte nuclear factor HNF-3β expressing endoderm (J), and alpha smooth muscle actin (αSMA)-positive mesodermal cells (K). Bar: 200 µm.

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