Figure 2 | Scientific Reports

Figure 2

From: E. coli allantoinase is activated by the downstream metabolic enzyme, glycerate kinase, and stabilizes the putative allantoin transporter by direct binding

Figure 2

AllB activates AllW-mediated allantoin transport. All strains used in this experiment were deleted for the allC gene (ΔallC). Squares and circles show the transport kinetics obtained for AllW in the presence of AllB in a ΔallB genetic background, expressed from the chromosome and from a plasmid, respectively. This shows complementation of the AllB stabilizing function. Higher transport activity is observed when the adjacent allW and allB genes are expressed together within the same operon and may result if complex formation occurs during or soon after synthesis, or if a stoichiometric relationship is required for maximal AllW activity.

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