Figure 1
From: Continuous fish muscle cell line with capacity for myogenic and adipogenic-like phenotypes
PCR confirmation of S. scombrus identity. (A) PCR amplification of Mack cell genomic DNA (Mack2 P3, Mack1 P55, Mack1 P4) with fish universal 5S primers and Scomber-specific Saba primers, with apparent band size matching expected size of 486 bp and 311 bp, respectively. The original gel is presented in Supplementary Fig. S1. (B) Comparison between Scomber species of Geneious Prime nucleotide alignment identity percentages of Sanger sequencing from Saba PCR reaction. Whiskers indicate min to max, n = 4; statistical significance calculated by one-way ANOVA with multiple comparisons between the control S. scombrus and the other species and indicated by asterisks, in which p < 0.0001 (****). (C) Section of Geneious Prime alignment of the consensus mackerel nucleotide sequence (n = 4) and the four Scomber species.
