Figure 2
From: Continuous fish muscle cell line with capacity for myogenic and adipogenic-like phenotypes
Spontaneous immortalization crisis event of Mack1 cells. (A–C) The progression of Mack1 cells through phase contrast imaging. Scale bars 200 μm. (A) Passage number 15, (B) passage number 42, (C) passage Number 84. Arrows in (B) indicate cells in crisis, as morphology is indicative of senescence. (D) Doubling time comparison between cells before crisis (passage numbers 12–26) and after crisis (passage numbers 114–128). Error bars indicate standard deviation, n = 15 distinct samples; statistical significance calculated by unpaired t-test and indicated by asterisks, in which p < 0.0001 (****). (E) Cell diameter comparison between cells before crisis (passage number 27) and after crisis (passage number 77). Error bars indicate standard deviation, n = 3 distinct samples; statistical significance calculated by unpaired t-test and indicated by asterisks, in which p < 0.01 (**).
