Figure 1 | Scientific Reports

Figure 1

From: RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding

Figure 1

Sample preparation workflow and data analysis pipeline. (A) RevGel-seq workflow steps for sample preparation to characterize scRNA-seq. Barcoded beads and cells are attached via a bifunctional chemical linker. These complexes are dispersed in the hydrogel in the liquid state and immobilized upon gelation. Following cell lysis, RNA molecules are captured on the barcodes. After reverse transcription, barcoded cDNAs are PCR-amplified. Further details are presented in “Methods”. (B) Workflow of the end-to-end data processing pipeline integrated in the Cytonaut platform. The pre-processing phase inputs the raw sequencing data (FASTQ files) and outputs quality indicators and count matrices, followed by the post-processing phase that inputs the count matrices to perform 2D embedding, cell clustering and differential gene expression. The Cytonaut Rover module enables interactive data visualization. Additional details in “Methods”.

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