Figure 4
Evaluation of large cell processing with cardiomyocytes. (A) Microscopic observations of cardiomyocytes following trypsinization (cell diameter of 20–35 µm). (B) Microscopic observations of cardiomyocytes following coupling procedure (red arrows indicate cells, green arrows indicate beads). (C) 2D projections of data from two subsamples (PCR tubes) from the same cardiomyocyte sample preparation. (D) Gene expression of RYR2 (cardiomyocyte enriched gene) and (E) CALD1 gene expression (smooth muscle cell enriched gene). (F) Estimated cell quantities obtained from numbers of cell-bead complexes (for all 10,000 input cells), observed bead recovery rate (for each PCR tube, max 12.5% for each of 8 PCR tubes), and transcript/gene counts per analyzed cell at average 50,000 raw reads per cell. Sequencing data was processed according to Methods, section “RevGel-seq sample preparation workflow” by the pre-processing analysis pipeline of Cytonaut, which automatically detects the analyzed cells.
