Figure 7

Validation of RNA seq analysis. (A) Bar graphs show log2 fold change of selected genes associated with Müller glia throughout the different stages of organoid formation and maturation. (B) The plot shows gene expression as log2 fold change as determined by qPCR and RNA-seq. The linear regression analysis and Pearson’s correlation co-efficient (Pearsons R = 0.56; R2 = 0.31; **P = 0.038) are shown.