Figure 3 | Scientific Reports

Figure 3

From: Iron response elements (IREs)-mRNA of Alzheimer's amyloid precursor protein binding to iron regulatory protein (IRP1): a combined molecular docking and spectroscopic approach

Figure 3

Fluorescence intensity of APP IRE mRNA binding to IRP1 protein was monitored quenching of the intrinsic protein fluorescence at 25 °C. Fluorescence intensity measurements of APP mRNA (—●—) binding to IRP1. For protein titration, the excitation maximum was 280 nm and emission maximum were monitored at 332 nm. The inset shows the corresponding Scatchard analyses of the titration data. The solid lines are fitted theoretical curves.

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