Figure 4 | Scientific Reports

Figure 4

From: Iron response elements (IREs)-mRNA of Alzheimer's amyloid precursor protein binding to iron regulatory protein (IRP1): a combined molecular docking and spectroscopic approach

Figure 4

Fluorescence intensity measurements for the binding of APP IRE mRNA to IRP1 protein in the presence of iron (anaerobic). APP mRNA (57-nucleotides) was melted and annealed prior to each titration and incubated with IRP1 protein. 100 nM IRP1 protein was incubated with varying amounts of APP IRE mRNA in the absence (—○—) and presence (—●—) of 50 µM iron. The excitation wavelength was 280 nm and emission wavelength were 332 nm. The solid lines are the fitted curves.

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