Figure 7 | Scientific Reports

Figure 7

From: Iron response elements (IREs)-mRNA of Alzheimer's amyloid precursor protein binding to iron regulatory protein (IRP1): a combined molecular docking and spectroscopic approach

Figure 7

Iron decreases the kinetic rates for the binding of APP IRE mRNA to IRP1. Representative kinetic data show the time-dependent decrease in fluorescence intensity caused by binding of APP mRNA and APP mRNA-Fe2+ with IRP1. APP mRNA and IRP1 concentration were 500 nM and 100 nM (final), and the Fe2+ concentration was 50 μM (final). The solid line represents the fitted curve for a single-exponential function. The residual for the exponential fits is shown in lower panels.

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