Figure 9 | Scientific Reports

Figure 9

From: Iron response elements (IREs)-mRNA of Alzheimer's amyloid precursor protein binding to iron regulatory protein (IRP1): a combined molecular docking and spectroscopic approach

Figure 9

Iron decreases APP IRE mRNA·IRP1 binding by changing kon and koff. APP mRNA·IRP1 complex was diluted in the stopped-flow cell with an equal volume of the titration buffer alone, curve (A) and 50 μM Fe2+ in the titration buffer, curve (B). Traces were best fit with the single-exponential function. The concentration of APP mRNA was 1 μM, and IRP1 was 0.1 μM after mixing. Data points were averaged from three independent experiments. The koff value was obtained from the Keq values and using Kd = koff/kon.

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