Table 1 Temperature-dependent dissociation constants (KD) for the interaction of APP IRE mRNA with IRP1 in the absence and presence of iron (anaerobic).

From: Iron response elements (IREs)-mRNA of Alzheimer's amyloid precursor protein binding to iron regulatory protein (IRP1): a combined molecular docking and spectroscopic approach

Complex

KD (nM)

5 °C

10 °C

15 °C

20 °C

25 °C

APP IRE mRNA-IRP1

8.1 ± 0.3

14.7 ± 0.2

20.2 ± 0.5

26.1 ± 0.4

32.0 ± 0.6

APP IRE mRNA-IRP1-Fe2+

19.3 ± 0.6

38.2 ± 0.5

61.1 ± 2.7

83.4 ± 3.4

111 ± 2.8

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