Figure 3

iTFAs specifically enhance activation of the ASK1-p38 MAP kinase pathway induced by eATP. (a–f) RAW264.7 (a–c) and BV2 (d–f) cells were pretreated with the indicated TFAs for 12 h at 200 µM, treated with ATP at 0.5 mM for 0, 15 or 30 min, and then subjected to immunoblotting with the indicated antibodies. Quantifications of immunoblots are shown in Supplementary Fig. S5. Original blots are presented in Supplementary Fig. S6.