Figure 5

TPI-FC. (a) Opto-fluidic recording system, made of a DH microscope in off-axis configuration combined to a microfluidic module. HWP, half wave plate; MO, microscope objective; M, mirror; PBS, polarizing beam splitter; BS, beam splitter; TL, tube lens; CMOS, camera. (b) Digital hologram taken from the holographic video sequence recorded by the system in (a). The 384 × 384 holographic ROIs containing the cells are highlighted. (c) Zoom-in of the red holographic ROI in (b). (d) 201 × 201 QPM numerically extracted from the holographic ROI in (c).